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Interfacial Enzyme Kinetics by Otto G. Berg, and Mahendra Kumar Jain Hardcover - 328 pages Shipped in CLICK HERE Cat.# JW-BCH7 $373.60 BUY Published:  2007   ISBN:  9780471493044

A vast number of biochemical reactions are catalysed by molecules fixed to the surface of membranes (or other biological structures) with molecules in the surrounding solution. The study of the mechanisms at these "Biointerfaces" are becoming increasingly important for the understanding of biological catalysts, such as enzymes. This project is the first book to deal with the physical and chemical principles of an emerging field of science, for which the authors have set the ground-work.

• The first book to deal with this newly emerging area.
• Concentrates on the chemical and physical foundation of enzyme catalysis
• Key area for the deeper understanding of biocatalytic processes
• Examples for proteins and nucleic acids, two central areas of biochemical and bioorganic research

Table of Contents:

Preface.
Theory Boxes.
List of Symbols.

1. Why Interfacial Enzymes?

Structural Diversity of Non-polar and Amphiphilic Solutes.
To Be or Not to Be in Water.
The Hydrophobic Effect.
Knotty Issues of Interfacial Enzymology.
Pathophysiology of Lipolytic Enzymes.
Secreted PLA2 (sPLA2) Prototype for Interfacial Enzymology.
Summary and Outlook: Towards the Paradign for Interfacial Enzymology.

2. Interface Phenomena: Accessibility and Exchange.

Aggregates and Dispersions.
Organized Micellar Aggregates in Aqueous Dispersions.
Amphiphile Organization and the Monomer - Micelle Equilibrium and Exchange.
The Concentration Issues in the Monomer - Aggregate Equilibria.
Exchange of Amphiphiles between Organized Interfaces.
Dispersed Phases.
Choice of Interface for Study of an Interfacial Enzyme.
Summary: Interface Determines the Accessibility and Replenishment Rate.

3. To Be or Not To Be: Dilemma for the Substrate in Solution.

Constrained Reaction Path for the Turnover in the Aqueous Phase.
Analysis of the Steady State Turnover Cycle in the Aqueous Phase.
Ascertaining the Reaction Path in the Aqueous Phase.
PAF-Acetylhydrolase Hydrolyzes the Monodisperse Substrate.
Equilibrium Partitioning and Binding to Aggregates.
Summary: The Equilibrium Dilemma.

4. Interfacial Processivity: Ensemble Behavior in the Scooting Mode.

Conceptualizing Interfacial Processivity.
The Fourfold Meaning of the Substrate Concentration.
Constraints for Defining the Variables for the Reaction Progress in the Scooting Mode.
Integrity of Vesicles during the Scooting Mode Reaction Progress.
Tests for the Scooting Mode reaction progress by PLA2.
Ensemble Behavior of the Binding of the Enzyme to Vesicles.
Summary: Ensemble Behavior in a Microscopically Heterogeneous Environment.

5. Analysis of the Processive Reaction Progress.

Michaelis - Menten Equation for the Turnover in the Interface.
Catalytic Parameters from the Integrated Processive Reaction Progress.
Additional Constraints for the Analysis of the Interfacial Turnover Events for PLA2.
Uses of the Primary Catalytic Parameters.
Catalytic Mechanism of PLA2.
The Quality-of-Interface Effect.
Apparent Interfacial Activation.
Hopping and the Fast Enzyme Exchange Limit.
Summary: Variations in the Processivity.

6. Detailed Balance Conditions for Interfacial Equilibria.

Binding of Ions to the Interface.
Equilibria for the Binding of the Enzyme to the Interface.
Effective Equilibrium Constants.
The Cofactor Binding Obligatory for the Substrate Binding.
Detailed Balance Conditions and Local Concentrations for Effective Ligand Binding.
Resolution of the Interfacial Constants for PLA2.
Detailed Balance Conditions for PLA2.
Summary: Primary Equilibrium Parameters for the Kinetic Path.

7. Rapid Substrate Replenishment in the Quasi-Scooting Mode.

Interfacial Catalytic Cycle Turnover in the Quasi-Scooting Mode.
Sparingly Soluble Substrates.
Reaction Rate with the Partitioned Substrate.
Interfacial Turnover by Triglyceride Lipase.
Lid on tl-Lipase Active Site.
Interfacial Allostery for the Quality-of-Interface effects.
Motifs for Close Contact of Proteins with the Interface.
Summary: Multiple States of the Enzyme at the Interface.

8. Interfacial Allostery.

Interfacial Catalytic Turnover in the Quasi-Scooting Mode.
The Apparent Rate Parameters for the Pig Pancreatic PLA2.
Ks Allosteric effects of the Interface.
Analysis of the Interfacila Anionic Charge Preference: kcatS.
The Structural Basis for the Anionic Interface preference.
Modelling the i-face of PLA2.
Site-Directed Mutagenesis to Discern Interactions Along the i-face.
Summary: Residues involved in Charge Compensation.

9. Inhibition: Specific or Nonspecific.

Specific Inhibitors.
Kinetic effects of Nonspecific Inhibitors.
Kinetic effects of the Interface-Based Competitive Inhibitors.
Influence of Cofactor on the Scooting Kinetics.
Effects of the Interface-Based Inhibitor on the Integrated Rate Equation in the Scooting Mode.
Partitioning of the Inhibitor and Substrate between the Interface and the Aqueous Phase.
Summary: Multiple Pathways for Reduction of the Observed Rate.

10. The Delay to the Steady State in the Reaction Progress.

Effects of the Accumulated products in the Zwitterionic Bilayers.
Model for the Delay Due to the Product Accumulation during the Reaction Progress on Phosphatidylcholine Vesicles.
Effect of the Accumulated Products on the Delay in the Monolayer Reaction Progress.
Summary: Activation by the Anionic Charge Induced by the Product Accumulation.

11. Nonidealities of the Dispersed Phases.

The Exchange Limit.
Exchange-Limited Kinetics of PLA2 in Detergent-Dispersed Mixed Micelles of Long Chain Phospholipids.
Effect of Bile Salts on the Pancreatic PLA2 Catalyzed Hydrolosis of Phosphatidylochines.
Kinetic Concerns for Interfaces of the Dispersed Phase.
The Nonideality Factor.
Summary: Nonidealities for Replenishment and Binding.

12. Effects of Reduction of Dimensionality.

Dissection of the Entropy Loss on Interface Binding.
Synergistic effects of the Interface on Enzyme-Substrate Binding, Local Concentration and Scaffolding.
Diffusion Times in 1D, 2D and 3D.
Rate Enhancement by Facilitated Diffusion in 2D.
Summary: Dimensionality effects on the Equilibrium and Diffusion.

Reference
Index.

Nanobiotechnology II: More Concepts & Applications by Chad A. Mirkin, and Christof M. Niemeyer Hardcover - 459 pages Shipped in CLICK HERE Cat.# JW-BCH8 $231.75 BUY Published:  2007   ISBN:  9783527316731

This second volume on a burgeoning field retains the proven concept of the spectacularly successful first one, extending and supplementing it. Individual sections are each dedicated to nanoparticles, nanostructures and patterns, nanodevices and machines, and nanoanalytics. Essential reading for an entire generation of scientists, this authoritative survey defines one of the most important new scientific fields to have emerged for many decades.

Table of Contents:

SELF-ASSEMBLY & NANOPARTICLES - NOVEL PRINCIPLES
Self-Assembled Artificial Transmembrane Ion Channels
Self-Assembling DNA Nanostructures for Patterned Molecular Assembly
Synthesis and Assembly of Nanoparticles and Nanostructures Using Bioderived Templates
Proteins and Nanoparticles: Covalent and Non-Covalent Conjugates
DNA Nanostructures for Patterned Molecular Assembly and Controlled Molecular Transport
Biocatalytic Growth of Nanoparticles for Sensors and Circuitry

NANOSTRUCTURES FOR ANALYTICS
Nanoparticles for Electrochemical Bioassays
Luminiscent Semiconductor Quantum Dots in Biology
Nanoscale Localized Surface Plasmon Resonance Biosensors
Cantilever Array Sensors for Bioanalysis
Scanning Ion Conduction Microscopy
Label-Free Nanowire and Nanotube Biomolecular Sensors for In Vitro Diagnostics of Cancer and other Diseases
Bionanarrays

NANOSTRUCTURES FOR MEDICINAL APPLICATIONS
Biological Barriers to Nanocarrier-Mediated Delivery of Therapeutic and Imaging Agents
Organic Nanoparticles: Adapting Emerging Techniques from the Electronics Industry for the Generation of Shape-Specific, Functionalized Carriers for Applications in Nanomedicine
Poly(amidoamine) Dendrimer-Based Multifunctional Nanoparticles
Nanoparticle Contrast Agents for Molecular Magnetic Resonance Imaging
Micro- and Nanoscale Control of Cellular Environment for Tissue Engineering
Diagnostics and Therapeutic Targeted Perfluorocarbon Nanoparticles

NANOMOTORS
Biological Nanomotors
Biologically Inspired Hybrid Nanodevices

Physical Chemistry for the Biological Sciences by Gordon G. Hammes Hardcover - 363 pages Shipped in CLICK HERE Cat.# JW-BCH9 $122.70 BUY Published:  2007   ISBN:  9780470122020

Methods in Biochemical Analysis Series, Volume 46, Biophysical Chemistry: Thermodynamics, Kinetics, and Spectroscopy provides an excellent resource for biochemistry and biological students and professionals who need to understand thermodynamics, kinetics, and spectroscopy.

Table of Contents:

PART I: THERMODYNAMICS.

1. Heat, Work and Energy.
2. Entropy and Free Energy.
3. Applications of Thermodynamics to Biological Systems.

PART II: CHEMICAL KINETICS.

4. Principles of Chemical Kinetics.
5. Applications of Kinetics to Biological Systems.

PART III: SPECTROSCOPY.

6. Fundamentals of Spectroscopy.
7. X-ray Crystallography.
8. Electronic Structure.
9. Circular Dichroism, Optical Rotary Dispersion, and Fluorescence Polarization.
10. Vibrations in Macromolecules.
11. Principles of Nuclear Magnetic Resonance and Electron Spin Resonance.
12.Applications of Magnetic Resonance in Biology.

PART IV: SPECIAL TOPICS.

13. Ligand Binding to Macromolecules.
14. Hydrodynamics.
15. Mass Spectrometry.

Appendix A: Useful Constants and Conversion Factors.
Appendix B: Structures of the Common Amino Acids at Neutral pH.
Appendix C: Common Nucleic Acid Components.
Appendix D: Standard Free Energies and Enthalpies of Formation at 298 K, I Atmosphere, pH 7, and 0.25 M Ionic Strength.
Appendix E: Standard Free Energy and Enthalpy Changes for Biochemical Reactions at 298 K, 1 Atmosphere, pH 7.0, pMg 3.0, and 0.25 M ionic Strength.

Index.

Bacterial and Eukaryotic Porins: Structure, Function, Mechanism by Roland Benz Hardcover - 382 pages Shipped in CLICK HERE Cat.# JW-BCH10 $231.75 BUY Published:  2006   ISBN:  9783527307753

This first book dedicated to the topic relates the known physiological functions of porins to their molecular structure and mechanism, as documented by various in vitro and in vivo methods, including the generation of null mutants in mice.

For the first time, it brings together biophysical evidence with studies performed in a cellular context, presenting a unified picture of the fundamental importance of porins for cellular function. With 16 contributions by an interdisciplinary team of leading porin researchers, this reference is essential reading for every molecular or structural biologist with an interest in this essential protein family.

Table of Contents:

Preface.
List of Contributors.

1. Regulation of Porin Gene Expression by the Two-Component Regulatory System EnvZ/OmpR
2. The Structures of General Porins
3. Role of Bacterial Porins in Antibiotic Susceptibility of Gram-negative Bacteria
4. Porins of the Outer Membrane of Pseudomonas aeruginosa
5. Regulation of Bacterial Porin Function
6. Reconstitution of General Diffusion Pores from Bacterial Outer Membranes
7. OmpA/OprF: Slow Porins or Channels Produced by Alternative Folding of Outer Membrane Proteins
8. Drug Efflux and Protein Export through Channel-tunnels
9. Structure-Function Relationships in Sugar-Specific Porins
10. Functional Reconstitution of Specific Porins
11. Energy-coupled Outer Membrane Iron Transporters
12. Structural and Functional Aspects of the Vitamin B12 Receptor BtuB
13. Structure and Function of Mitochondrial (Eukaryotic) Porins
14. Mitochondrial Porins in Mammals: Insights Into Functional Roles from Mutant Mice and Cells
15. Gene Family Expression and Multitopological Localization of Eukaryotic Porin/Voltage Dependent Anion-selective Channel (VDAC): Intracellular Trafficking and Alternative Splicing
16. Function of the Outer Mitochondrial Membrane Pore (Voltage-dependent Anion Channel) in Intracellular Signalling
Index.

Carrier-bound Immobilized Enzymes: Principles, Application and Design by Linqiu Cao, and Rolf D. Schmid Hardcover - 578 pages Shipped in CLICK HERE Cat.# JW-BCH11 $269.05 BUY Published:  2006   ISBN:  9783527312320

The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.
A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.

Table of Contents:

Foreword.

1. Introduction: Immobilized Enzymes: Past, Present and Prospects.
2. Adsorption-based Immobilization.
3. Covalent Enzyme Immobilization.
4. Enzyme Entrapment.
5. Enzyme Encapsulation.