COURSE OUTLINE: Overview: Key Concepts, Microscope Anatomy, Optical Path. Practical Aspects: Köhler Illumination I, Köhler Illumination II, Essential Details. Image Formation: Optics, Image Formation, Limits to Resolution.

• Explain the function of each part of the light microscope and show where it is located
• Define "resolution", "contrast", and "definition" as they relate to the light microscope. Explain which parts of the microscope affect each. Manipulate these parts
• The user will be able to summarize the optical path of light throughout the microscope from beginning to end
• The user can determine the magnification, numerical aperture, and optimum cover-slip thickness of an objective lens
• The user can perform each step in the process of Köhler illumination in order. They can also use the fine focus control to visualize the third dimension of a specimen
• The user can recognize when an objective lens is dirty and can perform this simple maintenance procedure
• The user can understand common lens aberrations as evidenced by choosing the best optics for a given application. The user will also be capable of critically evaluating manufacturer's specifications
• The user will have a spatially accurate, 3-D understanding of the three properties of light and how they relate to the light microscope