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Serous Fluid Module

Serous fluid production. Distinguishing transudates and exudates using laboratory testing.
Gross and microscopic examination of serous fluids

COURSE OUTLINE: Overview: Learning Objectives, Anatomy, Normal Production, Transudate, Exudate, Laboratory Findings, Clinical Causes. Gross Examination: Transudate, Turbid, Haemorrhagic, Clot, Bile Stained, Chylous. Procedures: Cell Count, Cyto-centrifuge, Wright Stain, Gram Stain. Microscopic Exam: Cell Types, Normal, Inflammation, Haemorrhage, Infection, Malignancy. Exams: Exam 1, Exam 2.

LEARNING OBJECTIVES

• Describe the normal production of serous fluids and the mechanisms of formation of transudates and exudates
• Distinguish transudates and exudates using lab testing, and understand their significance
• Describe the gross appearance of serous fluid specimens
• Describe the principles of manual chamber counts, cytocentrifuge preparation, and Wright staining
• Identify the major types of normal and abnormal cells in serous fluids and their significance

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Seminal Fluid Module

Semen analysis, including the determination of sperm concentration,
motility and morphology

COURSE OUTLINE: Overview: Learning Objectives, Anatomy, Specimen Collection Quality Control, References. Gross Examination: Fluid Characteristics, pH. Procedures: Prepare Wet Mount, Load Hemacytometer, Prepare Semen Smear, Staining. Microscopic Examination: Components of Fluid, Sperm Motility, Other Wet Mount Observations, Sperm Concentration, Sperm Morphology, Other Cell Types, Differential Count. Clinical Correlations: Semen Analysis, Sperm Function, Sperm Vitality, Anti-Sperm Antibodies, Post-vasectomy Evaluation, Prostatitis, CAVD, Ejaculatory Dysfunction.

LEARNING OBJECTIVES

• List the glandular contributions and cellular components of seminal fluid
• Describe the methods used to determine sperm concentration, motility and morphology
• List the abnormalities detected and reference ranges for semen analysis
• Discuss the goals and limitations of semen analysis in the clinical setting
   

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Cerebrospinal Fluid Module

Overview of the lumbar puncture procedure. Gross and microscopic
examination of cerebrospinal fluid

COURSE OUTLINE: Overview: Learning Objectives, Anatomy, Specimen Collection, Laboratory Findings, Microbiology. Gross Examination: Overview, Clarity, Traumatic Tap. Procedures Cell Count, Cyto-centrifuge, Wright Stain, Gram Stain, Electrophoresis, Latex Agglutination. Microscopic Examination: Cell Types, Normal, Inflammation, Haemorrhage, Infection, Malignancy. Exams: Exam 1, Exam 2.

LEARNING OBJECTIVES

• Describe the production and collection of cerebrospinal fluid (CSF)
• Describe the gross appearance of CSF specimens
• Describe the principles of manual chamber counts, cytocentrifuge preparation, and Wright staining
• Identify the major types of normal and abnormal cells in CSF and describe their significance

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Amniotic Fluid Module

The amniocentesis procedure and laboratory analysis of amniotic fluid

COURSE OUTLINE: Overview: Learning Objectives Specimen Collection. Gross Examination: Normal Fluid, Abnormal Fluids. Clinical Correlations: Screening for Birth Defects, Genetic Testing, Open Neural Tube Defects, Foetal Maturity, Haemolytic Disease.

LEARNING OBJECTIVES

• Describe the amniocentesis procedure and the most common indications for amniocentesis
• List the most common genetic abnormalities detected by cytogenetic analysis and their clinical features
• Describe the L/S ratio and other assays used to assess foetal lung maturity
• Describe the tests used to screen for open neural tube defects
• Describe haemolytic disease of the newborn (HDN) and how the severity of HDN is assessed in the lab
   

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